7MCP

Crystal structure of Staphylococcus aureus Cystathionine gamma-lyase, Holoenzyme dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.91956
Spacegroup name	P 43 2 2
Unit cell lengths	104.465, 104.465, 286.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.760 - 2.400
R-factor	0.1503
Rwork	0.149
R-free	0.17320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ixz
RMSD bond length	0.007
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.219	1.157
Rpim	0.054	0.291
Number of reflections	63162	3103
<I/σ(I)>	2.3	0.3
Completeness [%]	99.2
Redundancy	17.2
CC(1/2)	0.992	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	0.1 M Tris-HCl, pH 7.6, 1.4 M Tri Sodium Citrate