7MCN

Crystal structure of Staphylococcus aureus Cystathionine gamma-lyase, Holoenzyme with High HEPES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-05
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.91956
Spacegroup name	I 41 2 2
Unit cell lengths	104.282, 104.282, 287.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.520
R-factor	0.166
Rwork	0.164
R-free	0.18910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ixz
RMSD bond length	0.006
RMSD bond angle	0.904
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.580
High resolution limit [Å]	2.520	2.520
Rmerge	0.152	0.997
Rpim	0.033	0.211
Number of reflections	27451	1944
<I/σ(I)>	19.3	4
Completeness [%]	99.8
Redundancy	22.6
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1 M HEPES-Na, pH 7.5, 1.3 M Tri sodium Citrate