7MCB

Crystal structure of Staphylococcus aureus Cystathionine gamma lyase Holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-04
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.92010
Spacegroup name	I 41 2 2
Unit cell lengths	105.133, 105.133, 288.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.390 - 2.140
R-factor	0.163
Rwork	0.162
R-free	0.18380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ixz
RMSD bond length	0.007
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.190
High resolution limit [Å]	2.140	2.140
Rmerge	0.099
Number of reflections	45133	3211
<I/σ(I)>	23.7
Completeness [%]	99.8	97.5
Redundancy	18.7	18.1
CC(1/2)	0.999	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	0.1 M HEPES , pH 7.6 1.4 M Tri sodium Citrate