7MAE

HIV-1 Protease (I84V) in Complex with PU1 (LR3-46)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07812
Spacegroup name	P 21 21 21
Unit cell lengths	51.461, 58.693, 62.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.671 - 1.735
R-factor	0.1924
Rwork	0.191
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dgx
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.700	1.770
High resolution limit [Å]	1.735	1.735
Rmerge	0.094	0.682
Number of reflections	19643	1886
<I/σ(I)>	25	2.4
Completeness [%]	98.8	98.6
Redundancy	4.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	23-24% (w/v) Ammonium Sulfate, 0.1M Bis-Tris-Methane-HCl Buffer pH 5.5