7MAA

HIV-1 Protease (I84V) in Complex with UMass10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-11
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	51.183, 57.928, 62.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.202 - 1.933
R-factor	0.1846
Rwork	0.182
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dgx
RMSD bond length	0.005
RMSD bond angle	0.788
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.210	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.059	0.305
Number of reflections	13227	1283
<I/σ(I)>	24.5	4.3
Completeness [%]	91.8	89.2
Redundancy	4.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	23-24% (w/v) Ammonium Sulfate, 0.1M Bis-Tris-Methane-HCl Buffer pH 5.5