7M8Q

Complex structure of Methane monooxygenase hydroxylase and regulatory subunit with fluorosubstituted tryptophans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	102.493, 105.623, 299.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.330 - 2.080
R-factor	0.1835
Rwork	0.182
R-free	0.21220
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6vk5
RMSD bond length	0.003
RMSD bond angle	0.546
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc6_3830)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.620	2.120
High resolution limit [Å]	2.080	2.080
Rmerge	0.164	1.289
Number of reflections	193713	9550
<I/σ(I)>	7.5	1.3
Completeness [%]	99.2
Redundancy	5
CC(1/2)	0.990	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	TACSIMATE AND PEG3350