7M82

Human DNA Pol eta with dA-ended primer and dATP: in crystallo reaction for 300 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	98.678, 98.678, 82.163
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.060 - 2.070
R-factor	0.1696
Rwork	0.165
R-free	0.22460
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.007
RMSD bond angle	1.064
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.100	32.100	2.110
High resolution limit [Å]	2.070	5.610	2.070
Rmerge	0.126	0.036	0.617
Total number of observations	104997
Number of reflections	27709	1421	1405
<I/σ(I)>	9.1
Completeness [%]	99.8	98.8	100
Redundancy	3.8	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES