7M80

Human DNA Pol eta with dA-ended primer and dATP: in crystallo reaction for 100 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	98.844, 98.844, 82.037
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.100 - 1.980
R-factor	0.1612
Rwork	0.157
R-free	0.21420
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.300	32.300	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.079	0.024	0.567
Total number of observations	139977
Number of reflections	31730	1611	1571
<I/σ(I)>	8.8
Completeness [%]	99.7	97.8	100
Redundancy	4.4	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES