7M7Z

Human DNA Pol eta with dA-ended primer and dATP: in crystallo reaction for 40 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	98.779, 98.779, 82.064
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.080 - 1.820
R-factor	0.184
Rwork	0.180
R-free	0.22790
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.010
RMSD bond angle	1.322
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.860
High resolution limit [Å]	1.820	4.960	1.830
Rmerge	0.118	0.037	0.680
Number of reflections	40321	2037	2007
<I/σ(I)>	10.1
Completeness [%]	99.6	97.5	100
Redundancy	5.4	5.4	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES