7M6K

Crystal structure of the ARM domain from Drosophila SARM1 in complex with VMN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1
Unit cell lengths	39.065, 51.010, 76.560
Unit cell angles	103.49, 101.78, 96.07

Refinement procedure

Resolution	36.360 - 1.690
R-factor	0.1912
Rwork	0.190
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lcz
RMSD bond length	0.005
RMSD bond angle	0.850
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	1.720
High resolution limit [Å]	1.690	1.690
Rmerge	0.060	0.960
Rpim	0.040	0.590
Number of reflections	60345	2695
<I/σ(I)>	13.7
Completeness [%]	96.6
Redundancy	7.1
CC(1/2)	1.000	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20 % PEG 3350 and 0.2 M magnesium acetate tetrahydrate, pH 7.9