7M6B
The Crystal Structure of Mcbe1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-26 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.446, 73.750, 160.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.429 - 1.900 |
| Rwork | 0.206 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dpm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.529 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.429 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 50187 | 7031 |
| <I/σ(I)> | 12.8 | 1.61 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.39 | 4.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M Citric acid pH 4.0 and 1.0 M NaCl |
