7M5W

Crystal structure of the HMG-C1 domain of human capicua bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-31
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.890, 79.920, 106.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.840 - 2.950
R-factor	0.2111
Rwork	0.209
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jrp
RMSD bond length	0.003
RMSD bond angle	0.458
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.844	23.844	3.130
High resolution limit [Å]	2.950	8.360	2.950
Rmerge	0.083	0.028	0.506
Rmeas	0.088	0.030	0.536
Total number of observations	79037
Number of reflections	8838	426	1349
<I/σ(I)>	22.47	57.71	4.38
Completeness [%]	98.8	92.4	97.3
Redundancy	8.943	7.34	8.928
CC(1/2)	0.999	0.999	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	100 mM MES, 100 mM CaCl2, 100 mM NaCl, and 16% (w/v) PEG 8000