7M5F
Contact-dependent inhibition system from Serratia marcescens BWH57
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-14 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.691, 97.241, 44.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.640 - 1.590 |
| R-factor | 0.1417 |
| Rwork | 0.140 |
| R-free | 0.16670 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.909 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_3409) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.132 | 0.650 |
| Number of reflections | 35744 | 1750 |
| <I/σ(I)> | 20.82 | 2.98 |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 6.6 | 4.2 |
| CC(1/2) | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 8% v/v tacsimate pH 4.0, 20% w/v PEG 3350, cryo glycerol 15%, trypsin treated |
