7M57

Crystallographic structure of a primitive orthorhombic crystal form of STMV

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	CCD
Collection date	1998-06-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	192.080, 202.290, 177.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 4.000
Rwork	0.200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nia
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	4.250
High resolution limit [Å]	4.000	4.000
Rmerge	0.085	0.280
Number of reflections	48422	865
<I/σ(I)>	5.6	1.2
Completeness [%]	81.8	36
Redundancy	6.8	2.3
CC(1/2)	0.940	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	Crystals were grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs. The drops were 6 to 8 ul and composed of equal volumes of a 5 mg/ml virus stock solution buffered with 0.1 M phosphate at pH 6.5, and the reservoir. The reservoirs were 16% ammonium sulfate buffered at pH 6.0 with 0.1 M phosphate. Crystallization was a room temperature.