7M4N
Crystal structure of RBR E3 ligase RNF216 in complex with K63-linked di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953724 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.791, 84.414, 96.418 |
| Unit cell angles | 90.00, 102.15, 90.00 |
Refinement procedure
| Resolution | 42.210 - 2.520 |
| R-factor | 0.2214 |
| Rwork | 0.216 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RNF216/ubiquitin |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.598 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 2.620 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.153 | 0.933 |
| Rmeas | 0.208 | 1.286 |
| Rpim | 0.146 | 0.882 |
| Number of reflections | 22096 | 2331 |
| <I/σ(I)> | 5.6 | 1.2 |
| Completeness [%] | 98.8 | 91.7 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.987 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 0.2M ammonium sulfate, 30% PEG 4000 |
