7M4I

DNA Polymerase Lambda, dCTP:At Mn2+ Product State Ternary Complex, 420 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.750, 62.317, 142.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.723 - 2.005
R-factor	0.223
Rwork	0.222
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.070	0.056	0.823
Rmeas	0.078	0.063	0.921
Rpim	0.034	0.028	0.403
Total number of observations	161791
Number of reflections	33609	1784	1547
<I/σ(I)>	11.2
Completeness [%]	99.1	95	93.8
Redundancy	4.8	4.4	4.8
CC(1/2)		0.994	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG