7M43

DNA Polymerase Lambda, TTP:At Ca2+ Ground State Ternary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-31
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.988, 62.653, 140.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.023 - 1.978
R-factor	0.1944
Rwork	0.193
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.978	5.370	1.980
Rmerge	0.070	0.035	0.503
Rmeas	0.076	0.038	0.598
Rpim	0.029	0.015	0.317
Number of reflections	35088	1918	1618
<I/σ(I)>	10.6
Completeness [%]	99.3	98	92.8
Redundancy	6.5	6.7	3.2
CC(1/2)		0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG