7M3X
Crystal Structure of the Apo Form of Human RBBP7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.726, 88.775, 97.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.630 - 1.460 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.19250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cfs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.446 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.490 |
| High resolution limit [Å] | 1.460 | 3.960 | 1.460 |
| Rmerge | 0.074 | 0.035 | 0.777 |
| Rmeas | 0.078 | 0.037 | 0.829 |
| Rpim | 0.023 | 0.011 | 0.286 |
| Total number of observations | 727132 | ||
| Number of reflections | 68121 | 3687 | 3354 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 99.9 | 99.9 | 99.8 |
| Redundancy | 10.7 | 10.2 | 8.1 |
| CC(1/2) | 0.999 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 28%(w/v)PEG3350 MME, 0.2M NaCl, 0.1M Hepes pH7.8, 5% MPD, 5% Glycerol |
