7M2V

Crystallographic Structure of the Rhombohedral Crystal Form of STMV Grown from Chloride

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	173
Detector technology	CCD
Collection date	2002-06-15
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	165.634, 165.634, 433.315
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.800
Rwork	0.205
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oq8
Data reduction software	d*TREK
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.300	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.080	0.146
Number of reflections	345430	9879
<I/σ(I)>	13.4	2.7
Completeness [%]	84.0
Redundancy	4.5
CC(1/2)	0.998	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	279	Crystals grown by sitting drop vapor diffusion in Cryschem plates with 0.6 ml reservoirs and 6 to 8 ul drops. Drops were equal amounts of a 5 mg/ml virus stock solution buffered at pH 6.5 with 0.1 M phosphate, and the reservoir solution. The reservoir solution was 4% w/v NaCl in 0.1 M phosphate at pH 6.0. Crystallization was at 4 degrees C.