7M2H

Structural Snapshots of Intermediates in the Gating of a K+ Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.04
Spacegroup name	P 4
Unit cell lengths	154.321, 154.321, 74.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.801 - 2.642
R-factor	0.2379
Rwork	0.237
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.801	2.730
High resolution limit [Å]	2.640	2.640
Number of reflections	54531	4451
<I/σ(I)>	9.1	1
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.990	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25	293	50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate