7M1C

Crystal structure of the HCMV pentamer-specific antibody 1-32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2019-08-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	65.677, 65.677, 191.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.980 - 2.100
Rwork	0.268
R-free	0.29690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u2s
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.200	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	25404	13538
<I/σ(I)>	6	1.5
Completeness [%]	99.9	99.8
Redundancy	7.2
CC(1/2)	0.987	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2.0 M ammonium sulfate, 0.2 M sodium chloride, 5% isopropanol