Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.784, 116.784, 129.256 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.330 - 3.120 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pp9 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.330 | 3.232 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmerge | 0.146 | |
| Rmeas | 0.151 | |
| Rpim | 0.039 | 0.472 |
| Number of reflections | 18575 | 1809 |
| <I/σ(I)> | 18.33 | 1.66 |
| Completeness [%] | 99.9 | |
| Redundancy | 15.1 | |
| CC(1/2) | 0.999 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris 8.5, 200 mM Lithium Sulfate and 22% PEG 3,350 |
