Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.722, 115.722, 129.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.210 - 3.090 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pp9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.295 | 3.232 |
| High resolution limit [Å] | 3.090 | 3.120 |
| Rmerge | 0.161 | |
| Rpim | 0.046 | 0.583 |
| Number of reflections | 18823 | 1816 |
| <I/σ(I)> | 18.11 | 1.66 |
| Completeness [%] | 99.8 | 99.62 |
| Redundancy | 13 | |
| CC(1/2) | 0.998 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Bis-Tris pH 8.5, 200 mM Lithium Sulfate, and 22% PEG3350 |
