Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.964, 116.964, 129.733 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.435 - 3.190 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pp9 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.440 | 3.304 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.138 | |
| Rmeas | 0.153 | |
| Rpim | 0.066 | 0.605 |
| Number of reflections | 17505 | 1733 |
| <I/σ(I)> | 10.9 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.8 | 10 |
| CC(1/2) | 0.996 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris 8.5, 200 mM Lithium Sulfate and 22% PEG 3350 |
