7M0O

DGT-28 EPSPS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978560
Spacegroup name	P 21 21 21
Unit cell lengths	39.270, 56.720, 152.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.020 - 1.620
R-factor	0.195
Rwork	0.194
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gga
RMSD bond length	0.007
RMSD bond angle	0.834
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.095	1.648
High resolution limit [Å]	1.620	1.620
Rmerge	0.083	0.849
Rpim	0.048	0.470
Number of reflections	44171	2154
<I/σ(I)>	17.1	2.1
Completeness [%]	99.9	99.3
Redundancy	7.7	8
CC(1/2)	0.995	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	277	1.2M sodium/potassium phosphate