7LZP

LC/A-JPU-B9-JPU-A11-JPU-G11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 65 2 2
Unit cell lengths	132.604, 132.604, 413.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.060 - 2.860
R-factor	0.2365
Rwork	0.235
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v0a
RMSD bond length	0.003
RMSD bond angle	0.584
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	137.680	2.960
High resolution limit [Å]	2.860	2.860
Rpim	0.088	0.893
Number of reflections	50539	4409
<I/σ(I)>	12.2
Completeness [%]	99.6
Redundancy	9.7
CC(1/2)	0.998	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium cacodylate pH 6.5, 15 % ethylene glycol, 25 % PEG 4000