7LYU
Reelin repeat 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-30 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.953724 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.171, 104.059, 67.615 |
| Unit cell angles | 90.00, 104.11, 90.00 |
Refinement procedure
| Resolution | 45.120 - 3.000 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e26 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.895 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.120 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 16263 | 2620 |
| <I/σ(I)> | 5.5 | 1.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.985 | 0.496 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris pH 8, 35% PEG 1000 |
