7LXS
Structural and Biochemical Insight into Assembly of Molecular Motors Involved in Viral DNA Packaging
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-01 |
Detector | RIGAKU HyPix-3000 |
Wavelength(s) | 1.5 |
Spacegroup name | P 1 |
Unit cell lengths | 24.747, 50.761, 68.527 |
Unit cell angles | 90.00, 88.36, 90.11 |
Refinement procedure
Resolution | 24.740 - 2.210 |
R-factor | 0.14 |
Rwork | 0.140 |
R-free | 0.14200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lw0 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.740 | 2.290 |
High resolution limit [Å] | 2.206 | 2.210 |
Number of reflections | 15881 | 1287 |
<I/σ(I)> | 93.9 | |
Completeness [%] | 95.1 | |
Redundancy | 3.7 | |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 50mM MES pH 6.5, 10mM zinc chloride, 20% PEG 400 |