7LXF

ENAH EVH1 domain bound to peptide from protein PCARE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	P 61 2 2
Unit cell lengths	52.120, 52.120, 197.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.140 - 1.650
R-factor	0.2182
Rwork	0.217
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rd2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.140	45.140	1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.066	0.034	4.117
Rmeas	0.067	0.035	4.199
Total number of observations	742720
Number of reflections	20302	320	1449
<I/σ(I)>	26.94	94.78	0.79
Completeness [%]	99.9	99.1	99.3
Redundancy	36.584	28.366	25.983
CC(1/2)	1.000	1.000	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Tris pH 8.0, 3.30M NaCl