7LWH

Human neurofibromin 2/merlin residues 1-339 in complex with LATS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	52.241, 84.241, 95.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.280 - 1.606
R-factor	0.2064
Rwork	0.205
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zrk
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.300	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	55750	2710
<I/σ(I)>	18.5	2.3
Completeness [%]	99.0	97
Redundancy	13.5
CC(1/2)	0.999	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	600 mM imidazole (pH 6.5), 15% (w/v) polyethylene glycol 3,350