7LVS
The CBP TAZ1 Domain in Complex with a CITED2-HIF-1-Alpha Fusion Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-09-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.061, 50.112, 41.255 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 40.730 - 2.020 |
| R-factor | 0.2137 |
| Rwork | 0.211 |
| R-free | 0.24060 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.645 |
| Data scaling software | HKL-2000 |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.730 | 2.146 |
| High resolution limit [Å] | 2.000 | 2.019 |
| Number of reflections | 8465 | 562 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.878 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 6.87, 28% PEG 4000 |
