7LVL

Dihydrodipicolinate synthase bound with allosteric inhibitor (S)-lysine from Candidatus Liberibacter solanacearum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	210
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	C 1 2 1
Unit cell lengths	101.763, 133.484, 155.778
Unit cell angles	90.00, 100.45, 90.00

Refinement procedure

Resolution	46.202 - 2.010
R-factor	0.1704
Rwork	0.169
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7loy
RMSD bond length	0.007
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.240	2.082
High resolution limit [Å]	2.010	2.010
Number of reflections	135620	13469
<I/σ(I)>	13.45
Completeness [%]	99.9
Redundancy	4
CC(1/2)	0.954	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	20% w/v PEG3000, 0.1 M sodium citrate, pH 5.5