Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-05 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 143.263, 153.811, 87.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.300 |
| Rwork | 0.189 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pyz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.509 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.968 | 2.340 |
| High resolution limit [Å] | 2.299 | 2.300 |
| Number of reflections | 86779 | 4507 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 1.9 | 1.7 |
| CC(1/2) | 0.998 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 50 mM sodium citrate, pH 5.6, 2% Tacsimate, pH 5.0, 5% PEG3350 |
