7LU6

Crystal structure of the mouse Kirrel3 D1 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97917
Spacegroup name	P 63
Unit cell lengths	63.241, 63.241, 347.809
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.520 - 1.950
R-factor	0.1648
Rwork	0.160
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ltw
RMSD bond length	0.005
RMSD bond angle	0.684
Data reduction software	HKL-2000
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.114	0.853
Rmeas	0.130	0.970
Rpim	0.062	0.459
Number of reflections	57041	2865
<I/σ(I)>	14.6	1.9
Completeness [%]	99.9	99.8
Redundancy	4.4	4.4
CC(1/2)		0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M Sodium cacodylate, pH 6.5, 1.4 M Sodium acetate