7LU4
Crystal structure of bacterial glycyl tRNA synthetase in complex with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 238.580, 127.860, 133.060 |
| Unit cell angles | 90.00, 103.61, 90.00 |
Refinement procedure
| Resolution | 35.740 - 2.500 |
| R-factor | 0.2085 |
| Rwork | 0.207 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f5w |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.586 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.620 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.126 | |
| Rmeas | 0.134 | |
| Rpim | 0.045 | |
| Number of reflections | 132960 | 6548 |
| <I/σ(I)> | 14.6 | 1.1 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 17.1 | 15 |
| CC(1/2) | 0.990 | 0.452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 550 mM Magnesium formate, 100 mM sodium acetate, 1% sodium dextran sulfate 4000, 100 mM glycine pH 8.5, 3% sacarose |
