7LT8

Crystal structure of Ras suppressor-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-17
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97919
Spacegroup name	P 1 21 1
Unit cell lengths	31.851, 45.328, 83.052
Unit cell angles	90.00, 99.02, 90.00

Refinement procedure

Resolution	27.341 - 1.760
R-factor	0.175516614945
Rwork	0.173
R-free	0.21666
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.814
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELX
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.084	0.804
Number of reflections	22975	1136
<I/σ(I)>	36.52
Completeness [%]	97.1
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	296	PEG4000, lithium chloride