7LSW
Structure of Full Beta-Hairpin LIR from FNIP2 Bound to GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 202.510, 202.510, 202.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.120 - 3.050 |
| R-factor | 0.2289 |
| Rwork | 0.228 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hyo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.695 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.120 | 49.120 | 3.240 |
| High resolution limit [Å] | 3.050 | 9.150 | 3.050 |
| Rmerge | 0.494 | 0.104 | 9.422 |
| Rmeas | 0.498 | 0.105 | 9.481 |
| Rpim | 0.056 | 0.013 | 1.047 |
| Total number of observations | 2171504 | ||
| Number of reflections | 27664 | 1179 | 4363 |
| <I/σ(I)> | 11.5 | 0.8 | |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 78.5 | 67.1 | 81.3 |
| CC(1/2) | 0.999 | 0.999 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 2.0 M ammonium sulfate, 0.1 M HEPES pH 7.5, and 2% v/v PEG 550 MME |
