7LSI
Structure of KD035, a VEGFR2 monoclonal antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 62 |
| Detector technology | PIXEL |
| Collection date | 2016-02-02 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.164, 90.236, 81.529 |
| Unit cell angles | 90.00, 93.81, 90.00 |
Refinement procedure
| Resolution | 39.456 - 2.400 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.27420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n9g |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.460 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 50743 | 4626 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 96.5 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.900 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2M Na Phosphate monobasic monohydrate,20% PEG3350 |
