7LRY

Structure of HIV-1 Reverse Transcriptase in complex with DNA, (-)FTC-TP, and CA(2+) ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	90.256, 129.193, 131.320
Unit cell angles	90.00, 100.86, 90.00

Refinement procedure

Resolution	91.270 - 2.450
R-factor	0.1904
Rwork	0.190
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d3g
RMSD bond length	0.003
RMSD bond angle	0.727
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.270	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.169	5.562
Rmeas	0.174	5.717
Rpim	0.040	1.315
Number of reflections	108631	5368
<I/σ(I)>	10.1	0.8
Completeness [%]	100.0	100
Redundancy	18.3	18.5
CC(1/2)	0.997	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	10-12% PEG 8000, 50 MM BISTRIS-PROPANE PH 7.2, 50 MM AMMONIUM SULFATE, 5% GLYCEROL, 5% SUCROSE