7LRM
Structure of HIV-1 Reverse Transcriptase in complex with DNA, dCTP, and CA(2+) ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.663, 131.247, 137.491 |
Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
Resolution | 34.130 - 3.140 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.24370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d3g |
RMSD bond length | 0.002 |
RMSD bond angle | 0.582 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.200 |
High resolution limit [Å] | 3.140 | 3.140 |
Rmerge | 0.088 | 0.499 |
Rmeas | 0.097 | 0.590 |
Rpim | 0.039 | 0.305 |
Number of reflections | 53976 | 2194 |
<I/σ(I)> | 16.2 | 1.5 |
Completeness [%] | 98.0 | 79.8 |
Redundancy | 5.5 | 2.9 |
CC(1/2) | 0.914 | 0.574 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 10-12% PEG 8000, 50 MM BISTRIS-PROPANE PH 7.2, 50 MM AMMONIUM SULFATE, 5% GLYCEROL, 5% SUCROSE |