7LRH
C-terminal domain of RibD from Brucella abortus (5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate reductase)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 |
Unit cell lengths | 54.910, 56.700, 79.600 |
Unit cell angles | 95.99, 91.25, 113.16 |
Refinement procedure
Resolution | 50.360 - 1.920 |
R-factor | 0.2361 |
Rwork | 0.234 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3z |
RMSD bond length | 0.014 |
RMSD bond angle | 1.644 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.360 | 1.970 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.085 | 2.624 |
Rmeas | 0.089 | 2.738 |
Rpim | 0.026 | 0.779 |
Number of reflections | 65607 | 4379 |
<I/σ(I)> | 12.1 | 1 |
Completeness [%] | 98.1 | 96.9 |
Redundancy | 11.8 | 12.2 |
CC(1/2) | 0.999 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 294 | 12% (w/v) PEG 8000 + 0.1 M MES pH 6,2 + 0.2 M ammonium sulfate |