7LR8
Crystal structure of GH5_18-E153A from Streptomyces cattleya in complex with Manb1-4GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.869, 76.455, 79.920 |
| Unit cell angles | 90.00, 117.24, 90.00 |
Refinement procedure
| Resolution | 38.980 - 1.600 |
| R-factor | 0.1632 |
| Rwork | 0.162 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lr7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.826 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.980 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 106029 | 5114 |
| <I/σ(I)> | 12.9 | 1.4 |
| Completeness [%] | 98.5 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.999 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 28% PEG 4000, 0.2 M lithium sulfate, 0.1 M Tris pH 8.5 |
