7LR4
Complex of Fab 2/1.12 with domain 3 of P. berghei HAP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-26 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.880, 187.100, 74.370 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 47.685 - 2.100 |
| R-factor | 0.1899 |
| Rwork | 0.189 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6d |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.556 |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 1.530 |
| Number of reflections | 68138 | 4935 |
| <I/σ(I)> | 8.42 | 0.52 |
| Completeness [%] | 98.4 | 96.9 |
| Redundancy | 3.55 | 3.69 |
| CC(1/2) | 0.997 | 0.109 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 25% PEG3350, 0.2M Ammonium Sulfate, 0.1M Bis-Tris pH5.5 |
