7LR3
Complex of Fab 2/6.14 with domain 3 of P. berghei HAP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-26 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 78.250, 122.580, 168.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.767 - 2.800 |
| R-factor | 0.2541 |
| Rwork | 0.253 |
| R-free | 0.29180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6d |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.548 |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 40463 | 4935 |
| <I/σ(I)> | 12.8 | 0.52 |
| Completeness [%] | 99.3 | 96.9 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.997 | 0.109 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 10 mM HEPES, 20 mM Proline |
