7LQO
Crystal structure of a genetically encoded red fluorescent peroxynitrite biosensor, pnRFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-29 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.115834 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.203, 34.567, 88.815 |
Unit cell angles | 90.00, 110.90, 90.00 |
Refinement procedure
Resolution | 41.810 - 2.100 |
R-factor | 0.1908 |
Rwork | 0.188 |
R-free | 0.25390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f9g |
RMSD bond length | 0.008 |
RMSD bond angle | 1.571 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.810 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 13553 | 831 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 95.1 | |
Redundancy | 3.74 | |
CC(1/2) | 0.998 | 0.805 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288 | Sodium Phosphate, citric acid, PEG 400. |