7LQ2

Apo Rr RsiG- crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	59.479, 95.293, 117.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.457 - 1.850
R-factor	0.1745
Rwork	0.174
R-free	0.20220
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.460	1.920
High resolution limit [Å]	1.850	1.850
Rpim	0.032	0.374
Number of reflections	57371	5313
<I/σ(I)>	17.8	2.4
Completeness [%]	99.6
Redundancy	6.6
CC(1/2)	0.998	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12% isopropanol, 0.1 M HEPES pH 7.5, 0.1 M MgCl2