7LPO

Crystal structure of Cryptococcus neoformans sterylglucosidase 1 with tris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	150
Detector technology	PIXEL
Collection date	2019-06-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	98.597, 126.606, 126.655
Unit cell angles	90.00, 95.46, 90.00

Refinement procedure

Resolution	47.280 - 2.130
R-factor	0.159
Rwork	0.157
R-free	0.19760
Structure solution method	SAD
Data reduction software	Aimless
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.280	47.280	2.170
High resolution limit [Å]	2.130	11.670	2.130
Rmerge	0.111	0.033	0.593
Rmeas	0.133	0.039	0.757
Rpim	0.091	0.022	0.463
Number of reflections	171606	1095	7694
<I/σ(I)>	6.5	15	1.4
Completeness [%]	99.4	98.4	90.5
Redundancy	3.2	3.3	2.3
CC(1/2)	0.969	0.990	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	12 % PEG8000, 0.2M MgCl2, 0.1 M Tris 7.5