7LPH
APE1 Mn-bound product complex with abasic ribonucleotide DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-27 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.249, 61.570, 72.964 |
| Unit cell angles | 83.65, 78.15, 87.42 |
Refinement procedure
| Resolution | 24.720 - 1.990 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmeas | 0.080 | 0.574 |
| Number of reflections | 66383 | 2235 |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 96.4 | 88.5 |
| Redundancy | 5.6 | 3.3 |
| CC(1/2) | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200 mM MgCl2, 100 mM Sodium Citrate (pH 5), 10-15% PEG 20,000 |
