7LOZ
S-adenosylmethionine synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 122.600, 122.600, 288.835 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.090 - 2.250 |
| R-factor | 0.1906 |
| Rwork | 0.190 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pl7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.795 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.750 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 61889 | 4507 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 30.3 | |
| CC(1/2) | 0.998 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Bis-Tris 6.5, 15% ethylene glycol, PEG 3350 20% |
