7LOW

S-adenosylmethionine synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 61 2 2
Unit cell lengths	122.954, 122.954, 288.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.100 - 2.390
R-factor	0.184
Rwork	0.182
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pl7
RMSD bond length	0.003
RMSD bond angle	0.604
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.860	2.460
High resolution limit [Å]	2.390	2.390
Number of reflections	51892	4416
<I/σ(I)>	15.5
Completeness [%]	100.0
Redundancy	39.4
CC(1/2)	0.999	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Bis-Tris pH 6.5, 15% ethylene glycol, 20% PEG 3350